9YYA

Macrophage Migration Inhibitory Factor 1 from Necator Americanus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.920105
Spacegroup name	P 21 21 21
Unit cell lengths	124.632, 126.213, 155.766
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	98.255 - 2.098
Rwork	0.198
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.686
Data reduction software	XDS (Jan 10, 2022)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.0.8)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.255	2.134
High resolution limit [Å]	2.098	2.098
Rmerge	0.146	2.078
Rmeas	0.161	2.290
Number of reflections	143699	7143
<I/σ(I)>	7.4	1
Completeness [%]	99.9	100
Redundancy	5.7	5.7
CC(1/2)	0.995	0.345

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	60 %v/v Tacsimate (Precipitant)