9YXY

Crystal structure of EEPD1 EEP domain dimer at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979460
Spacegroup name	P 21 21 21
Unit cell lengths	38.943, 116.320, 121.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.240 - 2.000
R-factor	0.1963
Rwork	0.194
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.701
Data reduction software	XDS (20210323)
Data scaling software	Aimless (0.8.2)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.240	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.161	3.158
Rmeas	0.168	3.276
Rpim	0.045	0.863
Number of reflections	37041	2799
<I/σ(I)>	11.85	0.86
Completeness [%]	96.7	94.9
Redundancy	13.6	14.2
CC(1/2)	0.999	0.399

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	288	0.1 M Bis-Tris, pH 5.5, 25% PEG3350