9YWN
Protein Structure of the First Glycoside Hydrolase Family 30, Subfamily 12 Endoxylanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-16 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.9857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.307, 133.392, 137.700 |
| Unit cell angles | 90.00, 101.54, 90.00 |
Refinement procedure
| Resolution | 48.949 - 1.916 |
| Rwork | 0.169 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.597 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.210 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmeas | 0.131 | 1.157 |
| Rpim | 0.072 | 0.649 |
| Number of reflections | 239756 | 11542 |
| <I/σ(I)> | 9.4 | 0.89 |
| Completeness [%] | 99.6 | 96.2 |
| Redundancy | 3.3 | 2.9 |
| CC(1/2) | 0.994 | 0.403 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 0.17 M ammonium acetate, 0.085 M sodium acetate HCl pH 4.6, 25.5 percent PEG 4000 and 15 percent glycerol |
