9YW4

T cell receptor N17.2 complexed w/ HLA.A1 and NRAS peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92019
Spacegroup name	P 43 21 2
Unit cell lengths	126.452, 126.452, 174.741
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.990 - 3.100
R-factor	0.206
Rwork	0.203
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.078
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.310
High resolution limit [Å]	3.100	3.100
Number of reflections	26436	4702
<I/σ(I)>	8.4
Completeness [%]	91.2
Redundancy	11.9
CC(1/2)	0.995	0.226

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	1.6 M Ammonium sulfate, 100 mM Bicine 9