9YVZ

Crystal structure of red fluorescent protein mScarlet, 277 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	277
Detector technology	PIXEL
Collection date	2018-04-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.11
Spacegroup name	C 1 2 1
Unit cell lengths	84.128, 48.543, 59.860
Unit cell angles	90.00, 102.77, 90.00

Refinement procedure

Resolution	58.380 - 1.350
R-factor	0.1217
Rwork	0.120
R-free	0.14600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.077
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.380	1.370
High resolution limit [Å]	1.350	1.350
Number of reflections	51150	2130
<I/σ(I)>	5.5
Completeness [%]	98.7
Redundancy	3.2
CC(1/2)	0.985	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	Mother liquor 0.15 M (NH4)2SO4 24% PEG-3350 Soak 0.1 M HEPES, pH 7.5 0.15 M (NH4)2SO4 24% PEG-3350