9YVY

Crystal structure of red fluorescent protein mCherry, 277 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	277
Detector technology	PIXEL
Collection date	2018-04-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.11
Spacegroup name	P 1 21 1
Unit cell lengths	48.942, 43.855, 61.729
Unit cell angles	90.00, 110.68, 90.00

Refinement procedure

Resolution	57.750 - 1.660
R-factor	0.1644
Rwork	0.164
R-free	0.18280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.089
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.750	1.680
High resolution limit [Å]	1.660	1.660
Number of reflections	29201	1168
<I/σ(I)>	6.6
Completeness [%]	100.0
Redundancy	20
CC(1/2)	0.996	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	278	0.1 M Tris, pH 8.5 0.1 M sodium acetate 30% PEG-4K