9YVX

Crystal structure of red fluorescent protein mRouge, 277 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	277
Detector technology	PIXEL
Collection date	2018-04-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.11
Spacegroup name	P 1 21 1
Unit cell lengths	49.191, 43.489, 61.784
Unit cell angles	90.00, 111.86, 90.00

Refinement procedure

Resolution	45.650 - 1.380
R-factor	0.1267
Rwork	0.125
R-free	0.15130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.972
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.650	1.400
High resolution limit [Å]	1.380	1.380
Number of reflections	48458	2292
<I/σ(I)>	6.1
Completeness [%]	96.7
Redundancy	3.3
CC(1/2)	0.990	0.668

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		278	Mother liquor 0.1 M Tris pH 8.3 0.25 M MgCl2 20% PEG-4K Soak 0.1 M HEPES pH 7.5 0.25 M MgCl2 20% PEG-4K