9YV2
Crystal structure of the human DCAF1 WDR domain in complex with OICR-40151
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-08-03 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.794, 88.659, 73.394 |
| Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
| Resolution | 18.760 - 1.670 |
| R-factor | 0.22045 |
| Rwork | 0.219 |
| R-free | 0.25295 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.391 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.700 |
| High resolution limit [Å] | 1.670 | 4.530 | 1.670 |
| Rmerge | 0.076 | 0.028 | 0.809 |
| Rmeas | 0.087 | 0.033 | 0.934 |
| Rpim | 0.043 | 0.016 | 0.461 |
| Total number of observations | 277161 | ||
| Number of reflections | 69788 | 3598 | 3360 |
| <I/σ(I)> | 10 | ||
| Completeness [%] | 97.2 | 97.6 | 95.1 |
| Redundancy | 4 | 3.8 | 3.9 |
| CC(1/2) | 0.996 | 0.999 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30% PEG2000 Monoethyl Ether, 0.2 M Potassium Bromide |
