9YTZ

Structure of Ovine gammaherpesvirus 2 ORF73 LANA bound to importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95366
Spacegroup name	P 21 21 21
Unit cell lengths	78.533, 90.105, 100.421
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 2.850
R-factor	0.2299
Rwork	0.228
R-free	0.26470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.477
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.600	3.000
High resolution limit [Å]	2.850	2.850
Number of reflections	17239	2485
<I/σ(I)>	4.9
Completeness [%]	99.9
Redundancy	7.5
CC(1/2)	0.960	0.446

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.6M sodium citrate, 0.1M HEPES pH 7.0, and 10mM DTT