9YLO

BCL11A ZF2-3 in Complex with a DNA Sequence Observed in the Human Globin Locus Containing Motif TGTCCA (P21212 crystal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92019
Spacegroup name	P 21 21 2
Unit cell lengths	122.384, 29.964, 39.113
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.100 - 1.970
R-factor	0.2159
Rwork	0.214
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.524
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	2.000
High resolution limit [Å]	1.970	1.970
Rmerge	0.198
Rpim	0.065	0.847
Number of reflections	19231	1058
<I/σ(I)>	6.7	1.4
Completeness [%]	98.4	100
Redundancy	10.4	11.3
CC(1/2)	0.990	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	10% w/v PEG 20 000, 20% v/v PEG MME 500, 60mM sodium fluoride, 60mM sodium bromide, 60mM sodium iodide, 0.1M Bicine- TRIS-base (pH 8.5)