9YLL

BCL11A ZF4-6 in Complex with a DNA Sequence Observed in the Human Globin Locus Containing Motif TGTCCA (20-mer)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92026
Spacegroup name	P 43 21 2
Unit cell lengths	58.861, 58.861, 256.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.900 - 2.380
R-factor	0.2073
Rwork	0.205
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.531
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.900	2.760
High resolution limit [Å]	2.380	2.380
Rmerge	0.154
Rpim	0.032	0.478
Number of reflections	15340	792
<I/σ(I)>	13.7
Completeness [%]	46.5
Redundancy	24.7
CC(1/2)	0.998	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	292	20% w/v Polyethylene glycol 3,350 0.2M sodium chloride