9YKB

The von Willebrand factor A domain of human capillary morphogenesis gene II, fused to the 1TEL crystallization chaperone, Ala-Val linker variant, expressed with SUMO tag.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 65
Unit cell lengths	163.745, 163.745, 54.246
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	2.590 - 2.500
R-factor	0.181
Rwork	0.179
R-free	0.19380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.834
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	141.810	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.565	4.209
Rmeas	0.596	4.429
Rpim	0.187	1.372
Number of reflections	29090	2892
<I/σ(I)>	2.41	0.35
Completeness [%]	99.9	99.93
Redundancy	10	10.3
CC(1/2)	0.986	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1 M BisTrisPropane, 1.5M Ammonium Sulfate