9YK6

Crystal structure of anti-HCV human neutralizing antibody B107

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-14
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.920
Spacegroup name	P 21 21 21
Unit cell lengths	49.738, 73.318, 149.956
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.930 - 1.600
R-factor	0.2505
Rwork	0.250
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.506
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.930	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	73587	7212
<I/σ(I)>	10
Completeness [%]	100.0
Redundancy	6.9
CC(1/2)	0.990	0.310

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M potassium sulfate, 20%(w/v) polyethylene glycol 3350