9YJU
The structure of the periplasmic chaperone Skp from Neisseria meningitidis in space group P212121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-06-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.410, 118.960, 141.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.770 - 3.300 |
| R-factor | 0.2414 |
| Rwork | 0.239 |
| R-free | 0.29840 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.379 |
| Data scaling software | HKL-2000 |
| Phasing software | CRANK2 |
| Refinement software | PHENIX ((dev_4788: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.770 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 17881 | 2560 |
| <I/σ(I)> | 6.38 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 20 | |
| CC(1/2) | 0.868 | 0.040 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M potassium formate pH 7.3, 20% w/v PEG 3350 |
