9YJT

The structure of the periplasmic chaperone Skp from Neisseria meningitidis in space group P21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-12-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	84.461, 103.074, 128.721
Unit cell angles	90.00, 103.43, 90.00

Refinement procedure

Resolution	47.660 - 3.140
R-factor	0.2808
Rwork	0.278
R-free	0.33740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.519
Data reduction software	HKL-2000
Data scaling software	HKL-2000 (1.20_4459+SVN)
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.660	3.600
High resolution limit [Å]	3.140	3.400
Number of reflections	35885	5058
<I/σ(I)>	1
Completeness [%]	97.8	99.2
Redundancy	7
CC(1/2)	0.990	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M HEPES pH 7.5, 25% w/vPEG 1K