9YJS

Crystal structure of anti-HCV human broad neutralizing antibody B098

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	74.722, 74.722, 159.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.030 - 2.780
R-factor	0.235
Rwork	0.233
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.959
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.030	2.880
High resolution limit [Å]	2.780	2.780
Number of reflections	13457	652
<I/σ(I)>	9.07
Completeness [%]	99.9
Redundancy	17.6
CC(1/2)	0.990	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.08 M sodium acetate (pH 4.6), 0.16 M ammonium sulfate, 20 %(v/v) glycerol, 20 %(w/v) polyethylene glycol 4000