9YIP

Crystal structure of human IL-8 in a large unit cell

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97920
Spacegroup name	C 2 2 21
Unit cell lengths	44.881, 127.469, 210.291
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.730 - 2.200
R-factor	0.222
Rwork	0.221
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.467
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (2.0_5759)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.150	1.930
High resolution limit [Å]	1.890	1.890
Rpim	0.124
Number of reflections	48985	3130
<I/σ(I)>	3.4
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)	0.993	0.194

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.02 M Magnesium chloride hexahydrate, 0.05 M MOPS pH 7.0, 55% (v/v) Tacsimate pH 7.0, 0.002 M Hexamine cobalt (III) chloride