9YIO

Crystal structure of 5B3 Fab in complex with PvRipr EGF7-8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95365
Spacegroup name	P 21 21 2
Unit cell lengths	80.270, 81.746, 92.149
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.640 - 2.080
R-factor	0.2058
Rwork	0.204
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.615
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.640	2.130
High resolution limit [Å]	2.078	2.078
Rpim	0.065	0.611
Number of reflections	36982	4385
<I/σ(I)>	7.3
Completeness [%]	99.3
Redundancy	6.3
CC(1/2)	0.996	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	295	0.2M (NH4)2SO4, 20% PEG-3,350 and 0.1M Sodium Acetate buffer pH 4.4