9YI5

Macrophage Migration Inhibitory Factor 2 from Heligmosomoides polygyrus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-31
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.919716
Spacegroup name	P 63
Unit cell lengths	67.631, 67.631, 46.946
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.302 - 1.396
Rwork	0.149
R-free	0.17600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.660
Data reduction software	XDS (BUILT 20241002)
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.302	1.420
High resolution limit [Å]	1.396	1.396
Rmerge	0.113	1.761
Rmeas	0.116	1.822
Rpim	0.026	0.467
Number of reflections	24398	1231
<I/σ(I)>	15.514	1.417
Completeness [%]	100.0	100
Redundancy	20.13	15.11
CC(1/2)	0.999	0.348

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296.15	0.8 M Na2 Succ (Precipitant)