9YH8
M17T Human DJ-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.729 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.046, 75.046, 75.431 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.520 - 1.000 |
| R-factor | 0.1103 |
| Rwork | 0.110 |
| R-free | 0.13040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.017 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.720 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Number of reflections | 132397 | 6477 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 11.1 | |
| CC(1/2) | 1.000 | 0.443 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 19-22% PEG 4000, 200 mM MgCl2, and 100 mM Tris HCl, pH=8.5 |
