9YGS

Crystal structure of GMPPNP bound KRAS-Y71H in complex with RBD domain of CRAF(RAF1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 2
Unit cell lengths	67.265, 71.236, 44.896
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.480 - 1.680
R-factor	0.2022
Rwork	0.201
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.480	1.710
High resolution limit [Å]	1.680	1.680
Number of reflections	22863	2598
<I/σ(I)>	4.3
Completeness [%]	100.0
Redundancy	8.4
CC(1/2)	0.990	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	100 mM Potassium thiocyanate, 30% PEG monomethyl ether 2000.