9YG6

Measles Virus Fusion Glycoprotein Postfusion Core (T461A Variant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.91969
Spacegroup name	C 1 2 1
Unit cell lengths	103.067, 29.339, 71.878
Unit cell angles	90.00, 112.36, 90.00

Refinement procedure

Resolution	34.000 - 1.390
R-factor	0.2121
Rwork	0.210
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.340
Data reduction software	XDS (Jun 30, 2024 (BUILT 20241002))
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.000	1.600
High resolution limit [Å]	1.390	1.390
Rmerge	0.071	0.542
Rmeas	0.094	0.761
Rpim	0.060	0.533
Number of reflections	18584	929
<I/σ(I)>	8.7	2
Completeness [%]	88.4	59.5
Redundancy	4.3	3.1
CC(1/2)	0.998	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	281	30mM Sodium fluoride; 30mM Sodium bromide; 30mM Sodium iodide, 100mM Imidazole; MES monohydrate (acid), 12.5% v/v MPD; 12.5% PEG 1000; 12.5% w/v PEG 3350, (pH 6.5)