9YFP

Measles Virus Fusion Glycoprotein Postfusion Core (N462K Variant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92019
Spacegroup name	P 3 2 1
Unit cell lengths	47.725, 47.725, 71.398
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.020 - 1.860
R-factor	0.2595
Rwork	0.258
R-free	0.28830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.042
RMSD bond angle	0.547
Data reduction software	XDS (Jun 30, 2024 (BUILT 20241002))
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.020	1.890
High resolution limit [Å]	1.860	1.860
Rmerge	0.212	2.656
Rmeas	0.223	2.783
Rpim	0.067	0.829
Number of reflections	8314	400
<I/σ(I)>	10.3	1.5
Completeness [%]	99.7	99.3
Redundancy	19.9	20.8
CC(1/2)	1.000	0.423

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	281	30mM Sodium fluoride; 30mM Sodium bromide; 30mM Sodium iodide, 100mM Tris (base); BICINE, 20% v/v PEG 500* MME; 10 % w/v PEG 20000, (pH 8.5)