9YD9

Measles Virus Fusion Glycoprotein Postfusion Core (T461I Variant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92019
Spacegroup name	P 1 21 1
Unit cell lengths	35.086, 49.338, 55.661
Unit cell angles	90.00, 99.45, 90.00

Refinement procedure

Resolution	34.610 - 1.200
R-factor	0.1782
Rwork	0.177
R-free	0.19560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.052
RMSD bond angle	0.616
Data reduction software	XDS (Jun 30, 2024 (BUILT 20241002))
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.610	1.390
High resolution limit [Å]	1.200	1.200
Rmerge	0.071	1.128
Rmeas	0.083	1.317
Rpim	0.044	0.677
Number of reflections	37748	1887
<I/σ(I)>	10.8	1.4
Completeness [%]	92.1	51.7
Redundancy	7	7.3
CC(1/2)	0.999	0.412

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	282	20mM 1,6-Hexanediol; 20mM 1-Butanol; 20mM 1,2-Propanediol; 20mM 2-Propanol; 20mM 1,4-Butanediol; 20mM 1,3- Propanediol, 100mM Imidazole/ MES monohydrate (acid), 20% v/v PEG 500* MME; 10% w/v PEG 20000, (pH 6.5)