9YC3

Crystal structure of malaria transmission-blocking antigen PfHAP2 domain 3 in complex with nanobody WNb 334

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953740
Spacegroup name	P 32 2 1
Unit cell lengths	76.474, 76.474, 95.712
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.790 - 2.800
R-factor	0.2253
Rwork	0.224
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.597
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.790	2.970
High resolution limit [Å]	2.800	2.800
Rmerge	0.157	1.351
Rmeas	0.165	1.417
Number of reflections	8345	1325
<I/σ(I)>	12.95
Completeness [%]	99.9
Redundancy	11
CC(1/2)	0.999	0.593

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	293	0.1 M HEPES pH 7.7, 27% w/v PEG 3350