9YBK

[2ULA] Double crossover-like L-DNA motif containing a mobile junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92012
Spacegroup name	P 41
Unit cell lengths	35.985, 35.985, 102.023
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.400 - 2.630
R-factor	0.2262
Rwork	0.224
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.638
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.405	29.405	3.075
High resolution limit [Å]	2.613	7.054	2.613
Number of reflections	2012	202	202
<I/σ(I)>	8.2
Completeness [%]	84.8
Redundancy	5
CC(1/2)	0.995	0.995	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate