9YBJ

[2UDS] Double crossover-like DNA motif containing a mobile junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92021
Spacegroup name	P 43
Unit cell lengths	34.864, 34.864, 103.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.030 - 2.140
R-factor	0.268
Rwork	0.267
R-free	0.28470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.603
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.030	33.030	2.454
High resolution limit [Å]	2.140	6.957	2.142
Number of reflections	3349	197	197
<I/σ(I)>	12.2
Completeness [%]	83.6
Redundancy	8.9
CC(1/2)	0.969	0.858	0.448

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate