9YBI

Structure of the CBS pair domain from zebrafish magnesium transporter CNNM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	I 21 21 21
Unit cell lengths	86.907, 147.942, 151.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.900 - 3.000
R-factor	0.236
Rwork	0.235
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.551
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.904	3.050
High resolution limit [Å]	3.000	3.000
Number of reflections	18551	1746
<I/σ(I)>	2.1
Completeness [%]	99.5
Redundancy	20.5
CC(1/2)	0.956	0.362

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	1.1 M ammonium tartrate pH 6.5, 2% (w/v) benzamidine hydrochloride