9YBH

Anti HPA-1a Fab M-204 and integrin beta 3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 21 21 21
Unit cell lengths	52.386, 66.745, 221.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.980 - 2.080
R-factor	0.203
Rwork	0.201
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.923
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.950	2.190
High resolution limit [Å]	2.080	2.080
Number of reflections	47925	6882
<I/σ(I)>	8.9
Completeness [%]	100.0
Redundancy	10.7
CC(1/2)	0.997	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292.15	(NH4)2SO4, sodium acetate, PEG 4000