9YAC

Measles Virus Fusion Glycoprotein Postfusion Core (T461I/N462S/N465S Variant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92019
Spacegroup name	P 3
Unit cell lengths	50.255, 50.255, 71.643
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.660 - 1.630
R-factor	0.1794
Rwork	0.177
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.057
RMSD bond angle	0.661
Data reduction software	XDS (Jun 30, 2024 (BUILT 20241002))
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER (1.20.1_4487)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.660	1.610
High resolution limit [Å]	1.480	1.480
Rmerge	0.130	1.790
Rmeas	0.140	2.000
Rpim	0.060	0.880
Number of reflections	26224	1311
<I/σ(I)>	9.9	1.4
Completeness [%]	91.6	50
Redundancy	10.7	10.2
CC(1/2)	0.999	0.345

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	281	30mM Magnesium chloride hexahydrate; 30mM Calcium chloride dihydrate, 100mM Sodium HEPES/ MOPS (acid), 20% v/v PEG 500* MME; 10% w/v PEG 20000, pH 7.5