9Y9O

Measles Virus Fusion Glycoprotein Postfusion Core (G464W Variant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.91969
Spacegroup name	P 1 21 1
Unit cell lengths	28.851, 52.561, 70.588
Unit cell angles	90.00, 100.46, 90.00

Refinement procedure

Resolution	28.960 - 1.440
R-factor	0.1727
Rwork	0.171
R-free	0.20870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.055
RMSD bond angle	0.649
Data reduction software	XDS (Jun 30, 2024 (BUILT 20241002))
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER (1.21.2_5419)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.963	1.661
High resolution limit [Å]	1.439	1.439
Rmerge	0.120	0.713
Rmeas	0.163	0.993
Rpim	0.110	0.528
Number of reflections	24845	1242
<I/σ(I)>	5.7	1.5
Completeness [%]	90.6	41
Redundancy	3.7	3.3
CC(1/2)	0.996	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	281	10mM 1,6-Hexanediol; 10mM 1-Butanol; 10mM 1,2-Propanediol; 10mM 2-Propanol; 10mM 1,4-Butanediol; 10mM 1,3- Propanediol, 100mM Imidazole/ MES monohydrate (acid), 12.5% v/v MPD; 12.5% PEG 1000; 12.5% w/v PEG 3350