9Y8J

Crystal structure of the Kelch domain of human KLHL12 with compound 1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	H 3 2
Unit cell lengths	135.258, 135.258, 71.996
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.613 - 2.700
R-factor	0.2969
Rwork	0.293
R-free	0.32910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.952
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.613	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.117	0.532
Number of reflections	7049	624
<I/σ(I)>	11.3	1.5
Completeness [%]	99.9
Redundancy	9.7
CC(1/2)	0.995	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Bis-Tris pH 5.5, 0.2M MgCl2 and 25% PEG3350