9Y7K

Importin a2 in complex with Guertu virus non-structural S (NSs) NLS peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953725
Spacegroup name	P 21 21 21
Unit cell lengths	78.410, 89.648, 98.749
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.690 - 2.250
R-factor	0.1917
Rwork	0.191
R-free	0.20480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.489
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.690	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.039
Rmeas	0.114
Rpim	0.043
Number of reflections	36043	3075
<I/σ(I)>	10.6
Completeness [%]	99.9
Redundancy	6.8
CC(1/2)	0.998	0.617

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	0.70 M sodium citrate, 0.01 M DTT, 0.1 M HEPES, pH 7.0