9Y7G

CRYSTAL STRUCTURE OF THE A149T VARIANT OF SERINE HYDROXYMETHYLTRANSFERASE 8 FROM SOYBEAN CULTIVAR FORREST IN COMPLEX WITH PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2024-10-17
Detector	RDI CMOS_8M
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	175.184, 175.184, 183.569
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.750 - 1.870
R-factor	0.219
Rwork	0.217
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.871
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.750	1.890
High resolution limit [Å]	1.870	1.870
Rmerge	0.207	2.918
Rmeas	0.218	3.067
Rpim	0.067	0.938
Number of reflections	265645	12788
<I/σ(I)>	10.1
Completeness [%]	99.6	97.4
Redundancy	10.2	10.3
CC(1/2)	0.998	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium acetate trihydrate pH 7.0 12% w/v Polyethylene glycol 3,350