9Y74
[22L-7B C|A] 22 bp L-DNA tensegrity triangle that propagates via blunt-end stacking with C stacking on A at the interface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-07-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.919716 |
| Spacegroup name | P 63 |
| Unit cell lengths | 130.403, 130.403, 61.962 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.470 - 8.760 |
| R-factor | 0.2383 |
| Rwork | 0.237 |
| R-free | 0.31710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.619 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.470 | 56.466 | 11.764 |
| High resolution limit [Å] | 8.757 | 11.764 | 8.757 |
| Rmerge | 0.238 | ||
| Number of reflections | 443 | 222 | 221 |
| <I/σ(I)> | 3.3 | ||
| Completeness [%] | 84.9 | ||
| Redundancy | 4.7 | ||
| CC(1/2) | 0.979 | 0.978 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MOPS, 1.25 M magnesium sulfate |
