9Y6D

CRYSTAL STRUCTURE OF THE A149T VARIANT OF SERINE HYDROXYMETHYLTRANSFERASE 8 FROM SOYBEAN CULTIVAR FORREST IN COMPLEX WITH PLP-GLYCINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2024-10-17
Detector	RDI CMOS_8M
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	175.306, 175.306, 186.332
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.860 - 2.250
R-factor	0.2671
Rwork	0.265
R-free	0.31090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.862
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.860	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.175	1.379
Rmeas	0.179	1.421
Rpim	0.040	0.341
Number of reflections	156345	7641
<I/σ(I)>	20.8	3
Completeness [%]	100.0	99.9
Redundancy	20.3	17.5
CC(1/2)	0.999	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Ammonium tartrate dibasic pH 7.0 12% w/v Polyethylene glycol 3,350