9Y5G

Crystal Structure of rv-SNARE/sc-t-SNARE-5.s.8 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-02
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	43.182, 45.704, 341.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.340 - 2.100
R-factor	0.2298
Rwork	0.227
R-free	0.27810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.332
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.340	2.070
High resolution limit [Å]	2.020	2.020
Rmerge	0.250	2.143
Number of reflections	45885	3323
<I/σ(I)>	6.1	0.8
Completeness [%]	100.0	100
Redundancy	9	8.4
CC(1/2)	0.997	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Succinic acid pH 7.0 and 15% (w/v) PEG 3350