9Y5F

Crystal structure of rv-SNARE/sc-t-SNARE-5.s.8 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-02
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97934
Spacegroup name	P 41 21 2
Unit cell lengths	47.094, 47.094, 334.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.300 - 3.450
R-factor	0.2331
Rwork	0.227
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.318
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_4761)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	331.400	3.780
High resolution limit [Å]	3.450	3.450
Rmerge	0.174	0.531
Rpim	0.037	0.107
Number of reflections	5679	1293
<I/σ(I)>	15.3	8.6
Completeness [%]	99.8	100
Redundancy	23.8	26.1
CC(1/2)	0.999	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	293	0.2 M Ammonium acetate pH 7.1 and 20% w/v Polyethylene glycol 3,350