9Y55

A crystal structure of DUSP10 loop mutant I445A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	106.404, 110.048, 166.234
Unit cell angles	90.00, 90.20, 90.00

Refinement procedure

Resolution	166.230 - 3.502
R-factor	0.213
Rwork	0.211
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.629
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	166.233	3.520
High resolution limit [Å]	3.502	3.502
Rmerge	0.200	1.456
Number of reflections	46689	2456
<I/σ(I)>	6.5	1.5
Completeness [%]	96.0
Redundancy	6.9
CC(1/2)	0.989	0.403

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.1 M imidazole, pH 8.0, 2.5 M sodium chloride