9Y41
Crystal structure of hemagglutinin head domain from H1N1 Influenza A virus A/Victoria/2570/2019 bound to the 49_C09 antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-23 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.351, 256.212, 61.773 |
| Unit cell angles | 90.00, 99.68, 90.00 |
Refinement procedure
| Resolution | 36.660 - 2.890 |
| R-factor | 0.2378 |
| Rwork | 0.234 |
| R-free | 0.28280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.582 |
| Data reduction software | HKL-2000 (v722) |
| Data scaling software | HKL-2000 (v722) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rpim | 0.080 | 0.510 |
| Number of reflections | 25983 | 1844 |
| <I/σ(I)> | 9.7 | 1.4 |
| Completeness [%] | 87.9 | 81.5 |
| Redundancy | 3.9 | |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M Tris, pH 8.0, 65% (v/v) MPD |
