9Y40

Crystal structure of hemagglutinin head domain from H3N2 Influenza A virus A/New York/631/1996 bound to the 49_C09 antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	170.358, 170.358, 95.831
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.570 - 3.710
R-factor	0.2063
Rwork	0.203
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.541
Data reduction software	HKL-2000 (v722)
Data scaling software	HKL-2000 (v722)
Phasing software	PHASER
Refinement software	PHENIX (1.21)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.760
High resolution limit [Å]	3.700	3.700
Rpim	0.070	0.190
Number of reflections	16731	1600
<I/σ(I)>	7	2.5
Completeness [%]	100.0
Redundancy	18.2
CC(1/2)	1.000	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	4M sodium formate