9Y3X

Crystal structure of hemagglutinin from H1N1 Influenza A virus A/California/04/2009 bound to the 3_H2 antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-02
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 3 2 1
Unit cell lengths	130.894, 130.894, 142.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.250 - 3.390
R-factor	0.2099
Rwork	0.204
R-free	0.26080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.452
Data reduction software	HKL-2000 (7.22)
Data scaling software	HKL-2000 (7.22)
Phasing software	PHASER
Refinement software	PHENIX (1.21)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.460
High resolution limit [Å]	3.390	3.390
Rpim	0.040	0.620
Number of reflections	19112	1082
<I/σ(I)>	20.9	1.2
Completeness [%]	100.0	100
Redundancy	18.5	9.8
CC(1/2)	1.000	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Tri-potassium citrate, 20% (w/v) PEG3350, pH 8.3