9Y3W
Crystal structure of NRas-G12D in complex with GDP and compound 13
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-15 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92011 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.016, 46.237, 79.610 |
| Unit cell angles | 90.00, 89.19, 90.00 |
Refinement procedure
| Resolution | 29.390 - 1.560 |
| R-factor | 0.1754 |
| Rwork | 0.174 |
| R-free | 0.20930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.247 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.390 | 1.590 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 58131 | 1314 |
| <I/σ(I)> | 8.58 | |
| Completeness [%] | 99.8 | 99.54 |
| Redundancy | 3.6 | |
| CC(1/2) | 0.998 | 0.313 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1M Sodium Citrate pH5.5, 0.1M MgCl2, 16-22% Peg 4000 |
