9Y3C
[21-7B nWC] 21 bp tensegrity triangle with one set of Watson-Crick sticky ends, one of non-Watson-Crick sticky ends, and one of blunt ends
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 61 |
| Unit cell lengths | 125.139, 125.139, 187.813 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.960 - 6.430 |
| R-factor | 0.2119 |
| Rwork | 0.210 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.063 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 93.867 | 93.867 | 6.850 |
| High resolution limit [Å] | 6.427 | 16.529 | 6.427 |
| Number of reflections | 2894 | 205 | 208 |
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 84.2 | ||
| Redundancy | 20.5 | ||
| CC(1/2) | 1.000 | 1.000 | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MOPS, 1.25 M magnesium sulfate |
