9Y3B

[21-7B-001] 21 bp tensegrity triangle that propagates via blunt-end stacking on two sides and via sticky-end cohesion on one side

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	88.743, 104.798, 264.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.710 - 6.130
R-factor	0.3012
Rwork	0.299
R-free	0.35710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.598
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.602	65.602	7.588
High resolution limit [Å]	6.130	15.823	6.135
Number of reflections	1342	191	192
<I/σ(I)>	4.5
Completeness [%]	79.0
Redundancy	9.9
CC(1/2)	0.716	0.686	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate