9Y37
Rubredoxin from Pyrococcus Furiosus at 353K, formyl-Methionine N-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 353 |
| Detector technology | PIXEL |
| Collection date | 2025-01-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.022, 34.490, 42.623 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.830 - 1.840 |
| R-factor | 0.19128 |
| Rwork | 0.188 |
| R-free | 0.25284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.556 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.830 | 1.868 |
| High resolution limit [Å] | 1.837 | 1.837 |
| Rmerge | 0.223 | 1.652 |
| Rpim | 0.118 | 0.856 |
| Number of reflections | 4637 | 230 |
| <I/σ(I)> | 4 | 0.8 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 4.3 | 4.2 |
| CC(1/2) | 0.984 | 0.419 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | VAPOR DIFFUSION AGAINST 3.1 M NaCl with 3.6M Na,K PHOSPHATE, pH 8.0 |
