9Y1W

Crystal structure of NRas-G12D in complex with GDP and compound 27

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	42.460, 46.786, 79.939
Unit cell angles	90.00, 89.00, 90.00

Refinement procedure

Resolution	42.450 - 1.950
R-factor	0.1732
Rwork	0.170
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.188
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.450	2.040
High resolution limit [Å]	1.950	1.950
Number of reflections	32117	652
<I/σ(I)>	3.45
Completeness [%]	94.8
Redundancy	3.5
CC(1/2)	0.942	0.039

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.1M Sodium Citrate pH5.5, 0.1M MgCl2, 16-22% Peg 4000