9Y1L

Crystal Structure of Putative Restriction Endonuclease Domain-Containing Protein from Leptospirillum ferriphilum YSK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-10
Detector	SBC-3
Wavelength(s)	0.97931
Spacegroup name	P 31
Unit cell lengths	76.989, 76.989, 45.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.961 - 1.650
Rwork	0.154
R-free	0.17750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.488
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.061	0.295
Rmeas	0.067	0.322
Rpim	0.026	0.150
Number of reflections	36195	1568
<I/σ(I)>	31.7	5.7
Completeness [%]	98.8	85.9
Redundancy	6.2	4.4
CC(1/2)	0.996	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	Unknown