9Y0V

Crystal Structure of human MAIT A-F7 TCR-MR1*04 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	62.389, 140.991, 114.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.500 - 3.000
R-factor	0.1971
Rwork	0.195
R-free	0.24360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.467
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.720	3.120
High resolution limit [Å]	2.800	3.000
Number of reflections	39788	4483
<I/σ(I)>	6
Completeness [%]	99.9
Redundancy	3.5
CC(1/2)	0.956	0.478

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 6000, HEPES, Lithium Chloride