9Y0U

Crystal structure of human MAIT A-F7 TCR-MR1*03-5-OP-RU complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9536
Spacegroup name	C 1 2 1
Unit cell lengths	215.964, 69.919, 142.677
Unit cell angles	90.00, 104.33, 90.00

Refinement procedure

Resolution	47.790 - 2.100
R-factor	0.1618
Rwork	0.161
R-free	0.18740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.583
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.790	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	119799	5945
<I/σ(I)>	11.3
Completeness [%]	99.4
Redundancy	3.4
CC(1/2)	0.998	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, BTP, CaCl2